動物遺傳育種研究室

Department of Animal Science      http://www.as.nchu.edu.tw

Lab of Animal Genetics and Breeding (Molecular Biology)

研 究 室 成 員 Lab Members

 

教  授 黃木秋 博士

Mu-Chiou Huang,Prof.& Ph.D.

mchuang@mail.nchu.edu.tw                                

 

研究生

博士生 賴珊湖 陳佳萱 張慕慈

碩士生 陳子翰 簡肇宏 高瑋廷 李淑嫺 楊曉婷

陳品秀姜力嘉李威寰 謝佳吟 Pratthana   Jamwan 曲鳳祥

大學生 林逸勃 吳宸瑜

 

畢業生

博士班 林志生 顏念慈 劉炳燦 邱啟明 吳希天 洪炎明 吳建平 楊國泰 黃章文

碩士班 陳志正 黃秋容 林志生 吳希天 朱有田 陳慶任 廖乃祺 陳美惠 趙俊炫 羅偉男 蕭振文 何碧娟 洪炎明 掌慶健 張泰元 馬文輝 王得吉 楊國泰 吳美勤 陳億周 吳雅純 施彥仲 蔡佩宜 劉淑琳 何若澐 黃萬滄 董昭萍 黃章文 謝豐懇 簡嘉瑩 林明怡 李志明 廖俊欽 林佳瑜 林維楨 楊琇雅 辛彥龍 陳學毅 陳依婷 劉忠憲 陳嘉琪 呂智文 黃建勳 潘昭治 郭哲榮 張慕慈

研究助理 陳慧珊

前研究助理 簡嘉瑩  陳美卿  張永芬  林昱忻

楊國泰 林佳瑜 黃章文 王怡樺 詹雅婷

 

研究重點及成果

1.基因SNP鑑定—分析豬隻HFABP, CRC, RN, MYOG, ESR, PRLR, RPB4等肉質、繁殖、生長等性狀基因型，輔導農民選拔優良種豬。

2. Est序列及基因晶片分析—已利用本研究室DNA序列分析儀完成近8萬條豬與雞基因序列定序並利用晶片製備及分析儀尋找家畜禽經濟性狀之遺傳標記，以供選種之用。

3. DNA指紋鑑定—利用RAPD, RAMPO, VNTR, SSCP, AFLP等DNA指紋鑑定已成功的開發新的家畜禽種別、性別、親子鑑定等技術，並發表多篇論於國際著名期刊。

4. 粒線體DNA研究—分析家畜禽粒線體DNA D-loop區域及完整粒線體DNA序列，並發表論文於國際著名期刊。

5. 基因選殖、重組及轉殖—已成功選殖諸如牛、羊酪蛋白基因及hAT, HSA, hLZ, hSOD, hCAT,hGPX, gLF等多種基因，重組並轉殖入細胞、酵母菌及生產基因轉殖動物，從事生產高經濟價值重組基因蛋白質的研究。

Research works

1. SNP genotyping--- The SNP technique is used to investigate the genotypes of meat quality and reproductive traits of Taiwan black pigs. These genetic markers can be used for pig breeding to improve performances.

2. Est sequencing and cDNA Microarray--- We investigate the EST sequences in porcine and chicken. More than 80 thousands of EST sequences have been analyzed for bioinformatics analysis. Some students investigate the differential gene expression in animals using microarray technique.

3. DNA polymorphisms--- Animal RAPD, RAMPO, AFLP of genomic DNA are investigated. We have found some novel animal species and sex-specific sequences; it can be used for the species and gender determination.

4. Mitochondrial DNA--- The D-loop region and complete mtDNA sequences in domestic animals are investigated.

5. Gene manipulation---- Gene cloning, construction and transfer into Ecoli, yeast, mammalian cells to check gene expression or introduce the fusion genes into mouse embryos to produce transgenic mice. 

 

 

Genetic Analyzer

DNA定序儀

Microarray spotter

晶片製備儀

 

DNA microinjection

顯微注射

 

 

Fermentation

發酵

 

 

 

 

 

 

 

SNP genotyping of pigs  豬基因鑑定

 

 

cDNA chip

基因晶片

 

Transgenic mice

基因轉殖鼠

 

 

Hierarchical of differential expressed genes晶片分析

 

 

                                           

 

Publications

1.Yang, K.T., C.Y. Lin, J. S. Liou, Y. S. Fan, S. H. Chiou, C.W. Huang, C.P. Wu, E.C. Lin, C.F. Chen, Y. P. Lee, W.C. Lee, S.T. Ding, W.T.K. Cheng and M.C. Huang*. 2007. Differentially expressed transcripts in shell glands from low and high egg production strains of chickens using cDNA microarrays. Anim. Reprod. Sci. 101:113-124. SCI

2.Wu , C.P., Y.M. Horng,  R.T. Wang, K.T. Yang and M.C. Huang*. 2007. A novel sex-specific DNA marker in Columbidae birds. Theriogenology 67:328-333. SCI

3.Yen, N.T., C.S. Lin, C.C. Ju, S.C. Wang and M.C. Huang*. 2007. Mitochondrial DNA polymorphism and determination of effects on reproductive trait in pigs. Reprod. Domest. Anim. 42:387-392. SCI

4.  Huang, C.W., Y.S. Cheng, R. Rouvier, K.T. Yang, C.P. Wu, M.C. Huang*. 2007. AFLP fingerprinting for paternity testing in ducks. Br. Poultry Sci. 48:323-330. SCI

5.  Huang, C.W. and M.C. Huang*, 2007. A simple and rapid PCR-based method for ostrich sexing using micro amounts of DNA. Reprod. Domest. Anim. (In press) SCI

6.  Yang, K.T., C.Y. Lin, J.S. Liou, C.Y. Chien, C.P. Wu, C.W. Huang, B.R. Ou, C.F. Chen, Y.P. Lee, E.C. Lin, P.C. Tang, W.C Lee, S.T. Ding, W.T.K. Cheng and M.C. Huang*, 2007. Expressed transcripts associated with egg production trait in chicken ovarian follicles. Mol. Cell. Probe  SCI (In press)

7.Horng Y.M., C.P. Wu, Y.C. Wang and M.C. Huang*. 2006. A novel molecular genetic marker for gender determination of pigeons. Theriogenology 65:1759-1768. SCI

8.Weng, M.H., C.J. Chang, W.Y. Chen, W.K. Chou, H.C. Peh, M.C. Huang, M.T. Chen and H. Nagahata. 2006. Contribution of somatic cell-associated activation of plasminogen to caseinolysis within the goat mammary gland. J. Dairy Sci. 9:2025–2037 SCI

9.Wu, C.P., Y.M. Horng , K.T. Yang, C.W. Huang, and M.C. Huang*. 2006. Female-specific DNA sequences in ostriches. Mol. Cell Probe 20:307-310. SCI

10.     Wang J.W., Y.N. Jiang, C.Y. Huang, P.Y. Huang, M.C. Huang, W.T. Cheng, C.K. Shen and Y.T. Ju, 2006. Proliferin enhances microvilli formation and cell growth of neuroblastoma cells. Neurosci. Res. 56:80-90. SCI

11.     Yang K.T., C.Y. Lin, C.Y. Chien, C.P. Wu, C.W. Huang, B.R Ou, C.F. Chen, Y.P Lee, W.C. Lee, E.C. Lin, S.T. Ding, W.T.K. Cheng and M.C. Huang*. 2006. Effects of relative humidity, dot space, printing solution and slide coating on the dot morphology of gene chips. J. Chin. Soc. Anim. Sci. 35:145-151.

12.     Huang  C.W., S.C Liu, Y.H. Hu, K.T. Yang, C.P. Wu, Y.S. Cheng, R. Rouvier and M.C. Huang＊, 2006.The distinction of DNA fingerprints in Tsaiya ducks with different eggshell colors. J. Chin. Soc. Anim. Sci. 35:153-164.

13.  Huang, C.W., M.C. Huang＊, H.L. Liu, Y.S. Cheng, and R. Rouvier. 2006. Quantitative trait locus (QTL) detection for duration of fertility in common duck (Anas platyrhynchos) bred for mule ducks. Notes for Contributors of Symposium 2006 Scientific Cooperation in Agriculture between Council of Agriculture (Taiwan, R.O.C.) and Institut National de la Recherche Agronomique (France). pp.227-232.

14.     Huang, C.W., Y.S. Cheng, R. Rouvier, K.T. Yang, C.P. Wu, and M.C. Huang＊. 2006. The gene and genotype frequencies of Brown Tsaiya ducks (Anas platyrhynchos) selected for the duration of fertility. Notes for Contributors of Symposium 2006 Scientific Cooperation in Agriculture between Council of Agriculture (Taiwan, R.O.C.) and Institut National de la Recherche Agronomique (France). pp.233-236.

15.     Huang, S.Y., J.H. Lin, Y.H. Chen, C.K. Chuang, E.C. Lin, M.C. Huang, H.S. Sun and W.C. Lee. 2005. A reference map and identification of porcine testis proteins using 2-DE and MS. Proteomics 5: 4205–4212. SCI

16.     Horng, Y.M., Y.L. Hsin, C.P. Wu, C.Y. Lin and M.C. Huang*. 2005. Identification of animal-derived materials in ruminant feeds by random amplified polymorphic DNA fingerprinting. J. Agr. Assoc. China 6:54-63.

17.     Tian, S.Z., C.J. Chang, C.C. Chiang, H.C. Peh, M.C. Huang, J.W. Lee and X. Zhao. 2005. Comparison of morphology, viability, and function between blood and milk neutrophils from peak lactating goats. Can. J. Vet. Res. 69: 39-45. SCI

18.    Horng, Y.M., Y.T. Chen. , C.P. Wu., Y.S. Jea and M.C. Huang*. 2004. Cloning of Taiwan water buffalo male-specific DNA sequence for sexing Theriogenology 62:1536-1543. SCI

19.      Wu, H.T., C.K. Chou, and M. C. Huang*. 2004. Expression of human serum albumin in transgenic mice using goat b-casein/ human serum albumin fusion gene. Asian Austral. J. Anim. 17:743-749. SCI

20.     Chiu , C.M., S.B. Yae, M.Z. Tsai, S.P. Cheng and M.C. Huang*. 2004. Changes in number of granulosa cells, follicular fluid levels and diameter of oocyte during folliculogenesis in pre-pubertal gilts at marketing weight. Asian Austral. J. Anim. 17:1647-1651. SCI

21.     Horng, Y.M. and M.C. Huang*, 2003. Male-specific DNA sequences in pigs. Theriogenology 59:841-848. SCI

22.  Wu, H.T., C.S. Lin and M.C. Huang*, 2003. In vitro and ex vivo green fluorescent protein expression in alveolar mammary epithelial cells and mammary glands driven by the distal 5'-regulative sequence and intron 1 of the goat β-casein gene. Reprod. Fert. Develop. 15: 231-239. SCI

23.  Wu, H.T., C.K. Chou, .C.S. Lin and M.C. Huang*. 2003. Effects of glucose concentration on in vitro Fertilization in BAB/c Mice. Reprod. Domest. Anim. 38:470-474. SCI

24.  Huang, M.C.*, W.C. Lin,  Y.M. Horng , R. Rouvier and C.W. Huang., 2003. Female-specific DNA sequences in geese. Br. Poultry Sci. 44:359-364. SCI

25.  Huang, M.C*., Y.M. Horng , H.L. Huang, Y.L. Sin and M.J. Chen., 2003. RAPD fingerprinting for the species identification of animals. Asian Austral. J. Anim. 16: 1406-1410. SCI

26.  Liu, B.T., S.P. Cheng, M.C. Huang, J.Y.L. Lin, 2002. Serum progesterone changes in luteal cyclicity and duration of estrous cycle in Formosan sika dear (Cervus Nippon Taiouanus) hinds. Zool. Sci. 19: 1033-1037. SCI

27.   Lin, C.S., Y.C. Wu, Y.L. Sun and M.C. Huang*, 2002. Postnatal Expression of Growth/Differentiation Factor-8 (GDF-8) Gene in European and Asian Pigs. Asian Austral. J. Anim. Sci.. 15:1244-1249. SCI

28.  Wen J.S., C.J. Chang, H.P. Peh, S.L., M.C. Huang and X. Zhao, 2002. Apoptosis and oxidative stress of infiltrated neutrophils obtained from involuting mammary glands of goats during various stages of lactation. Am. J. Vet. Res. 63:241-246. SCI