動物蛋白質體學研究室-英文版

　

　

　

　

　 Laboratory for Animal Proteomics

　Chinese Version

Principal Investigator: San-Yuan Huang

Lab. Members:

PhD students: Hsin-Hsin Chen (PhD student in Dept. of Ved. Med., Co-advise with Prof Kwong-Chung Tung)

Tou-Huan Chen

MS students: Tung-Jen Lee, Si-Han Wang

Undergraduate students: Jun-Yi Liao, Hsiang-Ying Hsu, Kong-Ping Jih

　

Lab members (from left to right): H.H. Chen, T.J. Lee, S.H. Wang, S.Y. Huang, K.P. Jih, H.Y. Hsu, J.Y. Liao, and T.H. Chen

Graduated Members:

Undergraduate students: Yu-Chun Chen, Bin Chang (Now MS of Dept. Anim. Sci. & Technol., NTU)

Collaborators:

    Dr. Wen-Chuan Lee, Dr. Jyh-Hung Lin, Mr. You-Hai Kuo, Dr. Csaba Pribenszky; Dr. Ming F. Tam;

    Prof. Hsiao-Fang Sunny Sun; Dr.Chin-kai Chuang, Prof. En-Chung Lin, Dr. Ching-Fu Tu, Prof. Shiow-Her Chiou,

    Prof. Daw-Tung Lin, Prof. Feng-Pang Cheng, Prof. Yen-Pai Lee, Prof. Chih-Feng Chen, Prof. Ping-Chi Tang

　

Laboratory Introduction:

This Lab. was established in August 2006. The major mission was to continue the principal investigator's research topics in Animal Technology Institute Taiwan (ATIT) and to apply molecular genetics and proteomics related technical platform in studying and developing reproduction related molecular markers in livestock and poultry. The Lab. also participating the project for constructing a facility for isolated studies of GMO in transgenic livestock and poultry by playing roles in monitoring the proteomes of transgenic animals.

　

Research Topics:

1. Proteomic study and development of markers for reproduction and thermotolerance in livestock.

2. Monitoring of the proteome of transgenic animals.

3. Proteomic study on the chaperosome of spermatogenic cells.

4. Molecular mechanism of sperm motility/capacitation.

　

Research Grants Received:

Current projects:

1.Comparative proteomic studies on the testis proteins and functional analysis of chaperosomes in spermatogenic cells between avian and mammalian species

2008/08/01～2011/07/31

National Science Council [NSC97-2313-B-005-001-MY3]

Principal investigator: San-Yuan Huang      Co-Principal investigator: Ming F. Tam, Ching-kai Chuang.

　

2.Study and application of functional proteomics of elite boars

2008/01/01～2008/12/31

Council of Agriculture [97AS-1.2.1-AD-U1(4)]

Principal investigator: San-Yuan Huang Co-Principal investigator: Shiou-Her Chiou, Ching-kai Chuang.

　

3.Isolated Testing and Biosafety Assessment of Human FIX Transgenic Homozygote Sows and Hirudin Transgenic Pigs

2008/01/01～2008/12/31

Council of Agriculture [97AS-1.2.1-AD-U1(26)]

Principal investigator: Ching-Fu Tu Co-Principal investigator: San-Yuan Huang et al.

　

4.Development of DNA markers for elite elite boars

2008/01/01～2008/12/31

Council of Agriculture [97AS-1.2.1-AD-U1(5)]

Principal investigator: Ching-kai Chuang Co-Principal investigator: San-Yuan Huang, Wen-Chuan Lee.

Previous projects:

1. Stress protein profile in sperm of boars in Taiwan

1995/12/01～1996/06/30

Council of Agriculture [85AST-1.14-AID-23(1)]

Principal investigator: San-Yuan Huang

　

2. Study on the molecular markers for boar fertility

1996/07/01～1997/06/30

Council of Agriculture [87AST-1.5-AID-14(12)]

Principal investigator: San-Yuan Huang

　

3. Study on the molecular markers for boar fertility: development of DNA markers

1998/12/01～1999/6/30

Council of Agriculture [88AST-1.5-AID-08(2)]

Principal investigator: San-Yuan Huang

　

4. Effects of polymorphisms in hsp70.2 gene promoter on thermotolerance and growth performance in pigs.

1999/07/01～2000/12/31

Council of Agriculture [89AST-1.3-AID-6(9)]

Principal investigator: San-Yuan Huang; Co-Principal investigator: Wen-Chuan Lee

　

5. Establishment and Application of Porcine Testis Protein Database

2000/08/01～2002/07/31

National Science Council [NSC89-2313-B-059-045; NSC90-2313-B-059-002]

Principal investigator: San-Yuan Huang; Co-Principal investigator: Wen-Chuan Lee

　

6. Development of transgenic technology to improve thermoresistance in pigs

2002/01/01～2003/12/31

Council of Agriculture [91AS-3.1.3-FD-Z3]

Principal investigator: Wen-Chuan Lee ; Co-Principal investigator: San-Yuan Huang

　

7. Analysis of porcine reproductive proteome, and the development and application of protein chip

2002/01/01～2004/12/31

Council of Agriculture [91AS-1.1.3-AD-U1][92AS-3.1.1-AD-U1]

Principal investigator: San-Yuan Huang; Co-Principal investigator: Jyh-Hung Lin

　

8. The establishment of farm-isolated for testing transgenic livestock and avian

2002/08/01～2004/12/31

National Science Council, Council of Agriculture [NSC91-3112-P-059-002-Y]

Principal investigator: Chin-Fu Tu; Co-Principal investigator: Ping-Cheng Yang et al.

　

9. A proteomic study on the immunosupressive function of porcine Sertoli cells and their application in xeno-islet transplantation

2002/08/01～2005/07/31

National Science Council [NSC91-2313-B-059-005; NSC92-2313-B-059-002; NSC93-2313-B-059-001]

Principal investigator: San-Yuan Huang

　

10. Study on the association of disease resistance related candidate molecular markers and pig growth performance and establishment of detection platform

2005/01/01～2005/12/31

Council of Agriculture [94AS-4.1.1-AD-U1]

Principal investigator: San-Yuan Huang

　

11. Construction of a facility for isolated studies of GMO in transgenic livestock and poultry

2004/01/01～2008/12/31

Council of Agriculture [94AS-5.2.1-AD-U1]

Principal investigator: Ching-Fu Tu Co-Principal investigator: San-Yuan Huang et al.

　

12. Development and application of functional genomics and proteomics to improve reproductive performance of breeding pigs and Taiwan country chicken

2005/01/01～2005/12/31

Council of Agriculture [94AS-5.2.1-AD-U2]

Principal investigator: WTK Cheng Co-Principal investigator: San-Yuan Huang et al.

　

13. The effect of hydrostatic pressure on the motility and HSP level of boar spermatozoa before and after freezing

2005/06/01～2006/05/31

National Science Council [NSC94-2911-I-059-001]

Principal investigator: San-Yuan Huang Co-Principal investigator: Dr. Csaba Pribenzsky (Hungary)

　

14. Cloning and characterization of porcine HSP70t gene and its associated protein complex and biological function in spermatogenesis

2005/08/01～2007/07/31

National Science Council [NSC94-2313-B-059-004; NSC95-2313-B-005-081]

Principal investigator: San-Yuan Huang

　

15.The construction of facility for isolated studies of GMO in transgenic livestock and poultry, and evaluating the biosafety of human factor IX/ porcine lactoferin double transgenic ppigs

2008/01/01～2008/12/31

Council of Agriculture [97AS-1.2.1-AD-U1(29)]

Principal investigator: Ching-Fu Tu Co-Principal investigator: San-Yuan Huang et al.

　

　

16.Comparative proteomic studies on the testis proteins and functional analysis of chaperosomes in spermatogenic cells between avian and mammalian species

2007/08/01～2008/07/31

National Science Council [NSC96-2313-B-005-025]

Principal investigator: San-Yuan Huang      Co-Principal investigator: Ching-kai Chuang.

　

　

Recent Publications:

1. Huang, S.Y., J.H. Lin, Y.H. Chen, C.k. Chuang, Y.F. Chiu, M.Y. Chen, H.H. Chen, and W.C. Lee*, 2006. Analysis of chicken serum proteome and differential protein expression during development in single comb White Leghorn hens. Proteomics 6:2217-2224.

2. Huang, S.Y.*, H.L. Song, E.-C. Lin, W.C. Lee, J.C. Chiang, and H.L. Tsou, 2006. Association of polymorphisms in epidermal growth factor, prostaglandin-endoperoxide synthase 2 and prolactin receptor genes with semen quality in Duroc boars. Asian-Aust. J. Anim. Sci. 19:793-798.

3. Liu, S.H., H.H. Cheng, S.Y. Huang, P.C. Yiu, and Y.C. Chang*, 2006. Studying the protein organization of the postsynaptic density by a novel solid-phase- and chemical crosslinking-based technology. Mol. Cell. Proteomics 5:1019-1032.

4. Huang, S.Y., Y.H. Chen, I.C. Chen, L.L. Ho, C.-F. Tu.*, 2006. Protein expression of lymphocyte in HLA-DR transgenic pigs by a proteomic approach. Proteomics 6:5815-5825.

5. Liou, M.L., S.Y. Huang, J.H. Lin, Y.C. Liu, and W.C. Lee*, 2007. Association of serum protein levels with egg productivity in Taiwan red-feathered country chickens. Anim. Reprod. Sci. 100:158-171.

6. Hsieh, C.H., S.Y. Huang, Y.C. Wu, L.F Liu, C.C. Han, Y.C. Liu, and M.F. Tam*, 2007. Expression of proteins with dimethylarginines in Escherichia coli for protein-protein interaction studies. Protein Sci. 16:919-928.

7. Fan, Y.H., K.C. Chow, S.Y. Huang, L.M. Chi, C.J. Huang, and S.H. Chiou*, 2007. A missense polymorphism in porcine interferon-γ cDNA affects antiviral activity of the protein variant. Mol. Immunol. 44:3297-3304.

8. Hou, M.L.^#, S.Y. Huang^#, Y.K. Lai, and W.C. Lee*, 2008. Geldanamycin augments nitric oxide production and promotes capacitation in boar spermatozoa. Anim. Reprod. Sci. 104:56-68.

^# The authors contribute equally to this work.

9. Huang, S.Y.*, M.T. Chung, Y.C. Chen, H.L. Tsou, and H.L. Li, 2008. Association of polymorphism in alpha (1,2) fucosyltransferase gene with growth performance in two Western pig breeds in Taiwan. Livest. Sci. 114:336–340.

10. Huang, S.Y.*, C. Pribenszky, Y.H. Kuo, S.H. Teng, Y.H. Chen, M.T. Chung, and Y.F. Chiu, 2008. Hydrostatic pressure pre-treatment affects the protein profile of boar spermatozoa before and after freezing-thawing. Anim. Reprod. Sci. (In press)

Patents

1. Chen, M.Y., S.Y. Huang, H.L. Tsou, P.C. Yang, and W.C. Lee. Using polymorphism in porcine heat shock gene hsp70.2 to assess the growth performance. No. 221522, Republic of China.

2. Tzang, B.S., C.F. Tu, W.C. Lee, M.Y. Chen, S.Y. Huang, and J.H. Lin. Transgenic pig containing heat shock protein 70 transgene. USA patent No. US 7,115,796 B2.

　

　

　

Last uzpdated: 2008.09.15.