| 報告時間：2025-4-25 |
| 報告地點：Room 407 |
| 指導老師：Tsu-Tai Lee |
| 學生：Li-Ping Lo |
| 摘要 |
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Phytobiotics, known for their high bioactivity, have demonstrated beneficial effects on poultry by modulating immunity, reducing oxidative stress, promoting growth, and improving meat quality. As antibiotic-free and reduced-antibiotic farming becomes a growing trend, phytogenic additives show strong potential as alternatives to antibiotic growth promoters. Black garlic, produced through high-temperature self-fermentation of fresh garlic, exhibits significantly increased levels of functional sulfur compounds, particularly S-allyl cysteine (SAC), which is known for its immune-enhancing and health-promoting properties. This study aimed to investigate the effects of black garlic extract supplementation in broiler diets, applied in various forms (e.g., in drinking water, directly in feed, or with carriers), on growth performance and physiological responses. Results showed that broilers receiving black garlic extract had significantly higher body weight and weight gain at 35 days of age compared to the control group. Additionally, a significant increase in ileal muscle wall thickness was observed. At the molecular level, the expression of antioxidant-related genes Nrf2 and GCLC was significantly upregulated, while antioxidant proteins such as CAT and total antioxidant capacity were also enhanced, indicating improved oxidative defense. Regarding inflammatory markers, the expression of TNF-α, as well as the levels of IL-1β, IL-6, and IL-10, were significantly reduced, suggesting anti-inflammatory benefits. Microbiota analysis based on 16S rRNA sequencing revealed no significant differences in alpha diversity; however, principal component analysis showed distinct microbial distributions below the family level, indicating shifts in dominant bacterial taxa. According to Welch’s test, the control group had higher abundances of Nunduva fasciculata and Cutibacterium acnes, while Pseudoruminococcus massiliensis was significantly enriched in the black garlic extract group. Furthermore, functional pathway analysis revealed increased gene expression associated with vitamin K2 synthesis, γ-aminobutyric acid (GABA), ADP metabolism, and the citric acid cycle in the treatment group.In summary, black garlic extract supplementation enhances broiler growth performance and offers significant benefits in gut health and immune regulation. The observed improvements in microbial composition and metabolic indicators support the potential of black garlic extract, derived from garlic by-products, as a functional feed additive in poultry production. |
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