Poultry Breeding Laboratory

Chih-Feng CHEN, PhD
Distinguished Professor,
Email: cfchen@dragon.nchu.edu.tw
Tel: +886(0)4 22870613 ext.214
Fax: +886(0)4 22860265

Research topics
1. Genetic analysis of egg production traits using animal model;
2. Genetic diversity in local chicken;
3. Molecular markers-assisted selection in chicken.

 

 

 

 

 


Publications：
  1. Tan, F. J., D. C. Li, C. Kaewkot, H. I. Wu, K. Świąder, H. C. Yu, C. F. Chen, W. Chumngoen. 2021. Application of principal component analysis with instrumental analysis and sensory evaluation for assessment of chicken breast meat juiciness. Br Poult Sci. https://doi.org/10.1080/00071668.2021. 1955330.
  2. Mariadassou,M., M. Suez, S. Sathyakumar, A. Vignal, M. Arca, P. Nicolas, T. Faraut, D. Esquerré, M. Nishibori,A. Vieaud, C. F. Chen, H. M. Pham, Y. Roman, F. Hospital, T. Zerjal, X. Rognon, and M. Tixier-Boichard. 2021. Unraveling the history of the genus Gallus through whole genome sequencing. Mol Phylogenet Evol. 158:107044.
  3. Lien, C. Y., M. Tixier-Boichard, S. W. Wuan, and C. F. Chen. 2020. Identification of QTL and loci for egg production traits to tropical climate conditions in chickens. Livest. Sci. doi:10.1016/j.livsci.2020.103980.
  4. Chang, W. L., H. Wu, Y. K. Chiu, S. Wang , T. X. Jiang, Z. L. Luo, Y. C. Lin, A. Li, J. T. Hsu, H. L. Huang, H. J. Gu, T. Y. Lin, S. M. Yang, T. T. Lee, Y. C. Lai, M. Lei, M. Y. Shie, C. T. Yao, Y. W. Chen, J. C. Tsai, S. J. Shieh, Y. K. Hwu, H. C. Cheng, P. C. Tang, S. C. Hung, C. F. Chen, M. Habib, R. B. Widelitz, P. Wu, W. T. Juan, and C. M. Chuong. 2019. The making of a flight feather: bio-architectural principles and adaptation. Cell 179(6):1409-1423.
  5. Zhuang, Z.X., S. E. Chen, C. F. Chen, E. C. Lin, and S. Y. Huang. 2019.  Genome-wide association study on the body temperature changes of a broiler-type strain Taiwan country chickens under acute heat stress. J. Thermal Biol., 82:33-42.
  6. Widelitz, R.B., G. W. Lin, Y. C. Lai, J. A. Mayer, P. C. Tang, H. C. Cheng,T. X. Jiang, C. F. Chen, C. M. Chuong. 2019.  Morpho-regulation in diverse chicken feather formation: Integrating branching modules and sex hormone-dependent morpho-regulatory modules.  Dev. Growth Differ., 61(1):124-138.
  7. Tu, W. L., C. Y. Cheng, C. J. Chen, H. L. Chan, S. H. Wang, P. C. Tang, C. F. Chen, H. H. Chen, Y. P. Lee, S. E. Chen, S. Y. Huang. 2018.  Proteomic analysis of the hypothalamus of broiler-type Taiwan country chickens in response to acute heat stress. Anim. Sci., 89(10):1475-1485.
  8. Tu, W. L., C. Y. Cheng, C. J. Chen, H. L. Chan, S. H. Wang, P. C. Tang, C. F. Chen, Y. P. Lee, S. E. Chen, S. Y. Huang. 2018.  Annotation of differential protein expression in the hypothalami of layer-type Taiwan country chickens in response to acute heat stress. J. Therm. Biol., 77:157-172.
  9. Liu, W. B., H. C. Peh, C. K. Wang, M. C. Mangwe, C. F. Chen, H. I. Chiang. 2018.  Effect of seasonal changes on fertility parameters of Holstein dairy cow in subtropical climate of Taiwan. Asian-Australasian J. Anim. Sci., 31(6):820-826.
  10. Wang, S. H., C. Y. Cheng, C. J. Chen, H. L. Chan, H. H. Chen, P. C. Tang, C. F. Chen, Y. P. Lee, S. Y. Huang. 2018.  Acute heat stress changes protein expression in the testes of a broiler-type strain of Taiwan Country chickens. Animl Biotechnol., 19:1-17.
  11. Cheng, C. Y., W. L. Tu, C. J. Chen, H. L. Chan, C. F. Chen, H. H. Chen, P. C. Tang, Y. P. Lee, S. E. Chen, S. Y. Huang. 2018.  Functional genomics study of acute heat stress response in the small yellow follicles of layer-type chickens. Sci. Rep., 22:8(1):1320.
  12. Chumngoen, W., C. F. Chen, F.J. Tan. 2018.  Effects of moist- and dry-heat cooking on the meat quality, microstructure and sensory characteristics of native chicken meat. Anim. Sci. J., 89(1):193-201.
  13. Cheng, P. L., H. R., Wu, C. Y. Li, C. F. Chen, H. C. Cheng. 2017.  Characterization of the testicular regeneration potential in premature cockerels. J. Reprod. Dev., 63(6):563-570.
  14. Lien, C. Y., M. Tixier-Boichard, S. W. Wu, W. F. Wang, C. S. Ng, C. F. Chen.  2017. Detection of QTL for traits related to adaptation to sub-optimal climatic conditions in chickens. Genet. Sel. Evol., 49(1):39.
  15. Chumngoen, W., C. F. Chen, F. J. Tan. 2016.  Influences of end-point heating temperature on the quality attributes of chicken meat. Brit. Poul. Sci., 57(6):740-750.
  16. Pham, M. H., X. H. Tran, C. Berthouly-Salazar, M. Tixier-Boichard, C. F. Chen, Y. P. Lee. 2016.  Monitoring of genetic diversity in Taiwan conserved chickens assessed bypedigree and molecular data. Livestock Sci. 184:85-91.
  17. Cheng, C. Y., W. L. Tu, S. H. Wang, P. C. Tang, C. F. Chen, H. H. Chen, Y. P. Lee, S. E. Chen, and S. Y. Huang. 2015.  Annotation of Differential Gene Expression in Small Yellow Follicles of a Broiler-Type Strain of Taiwan Country Chickens in Response to Acute Heat Stress. , PLoS One (11):e0143418. doi: 10.1371.
  18. Ng, C. S., C. K. Chen, W. L. Fan, P. Wu, S. M. Wu, J. J. Chen, Y. T. Lai, C. T. Mao, M. Y. Lu, D. R. Chen, Z. S. Lin, K. J. Yang, Y. A. Sha, T. C. Tu, C. F. Chen, C. M. Chuong, and W. H. Li. 2015.  Transcriptomic analyses of regenerating adult feathers in chicken. BMC Genomics, 6;16(1):756. doi: 10.1186/s.
  19. Tu, W. L., C. Y. Cheng, S. H. Wang, P. C. Tang, C. F. Chen, H. H. Chen, Y. P. Lee, S. E. Chen, and S. Y. Huang. 2015.  Profiling of differential gene expression in the hypothalamus of broiler-type Taiwan country chickens in response to acute heat stress. Theriogenology, S0093-691X(15)00499-9. doi: 10.1016/j..
  20. Wang, S. H., C. Y. Cheng, P. C. Tang, C. F. Chen, H. H. Chen, Y. P. Lee, and S. Y. Huang. 2015.  Acute heat stress induces differential gene expressions in the testes of a broiler-type strain of Taiwan country chickens. PLoS One, 1;10(5):e0125816. doi: 10.1371.
  21. Chen, C. F., J. Foley, P. C. Tang, L. Ang, T. X. Jiang, P. Wu, R. B. Widelitz, and C. M. Chuong. 2015.  Development, regeneration and evolution of feathers. Annu. Rev. Anim. Biosci., 3:169-195.
  22. Cheng, C. Y., P. R. Chen, C. J. Chen, S. H. Wang, C. F. Chen, Y. P. Lee, and S. Y. Huang. 2015. Differential protein expression in chicken spermatozoa before and after freezing-thawing treatment. Anim. Reprod. Sci. , 152:99-107.
  23. Cheng,C. Y., P. R. Chen, C. J. Chen, S. H..Wang, C. F. Chen, Y. P. Lee, S. Y. Huang. 2014.  Differential protein expression in chicken spermatozoa before and after freezing–thawing treatment. Anim. Reprod. Sci., 152:99-107.
  24. Wang, Y. P., M. K. Hsieh, D. H. Tan, J. H. Shien, S. C. Ou, C. F. Chen, and P. C. Chang. 2014. The haemagglutinin of Avibacterium paragallinarum is a trimeric autotransporter adhesin that confers haemagglutination, cell adherence and biofilm formation activities. Vet. Microbiol., 174:474-482.
  25. Ng, C. S., P. Wu, W. L. Fan, J. Yan, C. K. Chen, Y. T. Lai, S. M. Wu, C. T. Mao, J. J. Chen, M. Y. Lu, M. R. Ho, R. B. Widelitz, C. F. Chen, C. M. Chuong, and W. H. Li . 2014. Genomic organization, transcriptomic analysis, and functional characterization of avian α-and β-keratins in diverse feather forms. Genome Biol Evol. , 6(9):2258-2273.
  26. Wang, S. H., C. Y. Cheng, C. J. Chen, H. H. Chen, P. C. Tang, C. F. Chen, Y. P. Lee, and S. Y. Huang. 2014.  Changes in protein expression in testes of L2 strain Taiwan country chickens in response to acute heat stress . Theriogenology, 82(1):80-94.
  27. Fan, W. L., C. S. Ng, C. F. Chen, M. Y. J. Lu, Y. H. Chen, C. J. Liu, S. M. Wu, C. K. Chen, J. J. Chen, C. T. Mao, Y. T. Lai, W. S. Lo, W. H. Chang, and W. H. Li. 2013. Genome-wide patterns of genetic variation in two domestic chickens. Genome Biol. Evol. 5(7):1376-1392.
  28. Pham, M. H., W. H. Chang, C. Berthouly-Salazar, D. Y. Lin, S. Yungrahang, C. C. Wang, Y. P. Lee, M. Tixier-Boichard, and C. F. Chen*. 2013. Genetic characterization of Taiwan commercial native chickens ascertained by microsatellite markers. J. Poult. Sci. 50(4):290-299.
  29. Pham, M. H., C. Berthouly-Salazar, X. H..Iran, W. H. Chang, R. P. Crooijmans, D. Y. Lin, V. T. Hoang, Y. P. Lee, M. Tixier-Boichard, and C. F. Chen*. 2013. Genetic diversity of Vietnamese domestic chicken populations as decision-making support for conservation strategies. Anim. Genet. 44(5):509-521.
  30. Lin, S. J., J. Foley, T. X. Jiang, C. Y. Yeh, P. Wu, A. Foley, C. M. Yen, Y. C. Huang, H. C. Cheng, C. F. Chen, B. Reeder, S. H. Jee, R. B. Widelitz, and C. M. Chuong. 2013. Topology of feather melanocyte progenitor niche allows complex pigment patterns to emerge. Science 340(6139):1442-1445.
  31. Chazara, 0., C. S. Chang, N. Bruneau, K. Benabdeljelil, J. C. Fotsa, B. B. Kayang, N. E. Loukou, R. Osei-Amponsah, V. Yapi-Gnaore, I. A. Youssao, C. F. Chen, M. H. Pinard-van der Laan, M. Tixier-Boichard, and Bed’hom B. 2013. Diversity and evolution of the highly polymorphic tandem repeat LEI0258 in the chicken MHC-B region. Immunogenetics 65(6):447-459.
  32. Wang, S. H., C. Y. Cheng, P. C. Tang, C. F. Chen, H. H. Chen, Y. P. Lee, and S. Y. Huang. 2013. Differential gene expressions in testes of L2 strain Taiwan country chicken in response to acute heat stress. Theriogenology 79:374-382.
  33. 林旻蓉、張伸彰、王志華、賈玉祥、范揚廣、陳志峰、李淵百。 2013。紅羽土雞的日間作息、鬥爭及性行為之比較。畜產研究 46:41-50。
  34. Chu, P. T., C.S. Lin, W. J. Chen, C. F. Chen, and H.W. Wen. 2012. Detection of gliadin in foods using a quartz crystal microbalance biosensor that incorporates goldnanoparticles. J Agric Food Chem., 4;60(26):6483-6492.
  35. Ng, C. S., P. Wu, J. G. Foley, A. Foley, M. McDonald, S. M. Leal, W. T. Juan, C. J. Huang, Y. T. Lai, W. S. Lo, C. F. Chen, R. B. Widelitz, P.I. Patel, W.H. Li, C. M. Chuong. 2012. Feather frizzling is due to a keratin mutation that causes a defective rachis. PLoS Genetics. Jul;8(7):e1002748.
  36. Pan, Y. E., Z. C. Liu, C. J. Chang, Y. L. Xie, C. Y. Chen, C. F. Chen, R. L. Walzem, and S. E. Chen. 2012. Ceramide accumulation and up-regulation of proinflammatory interleukin-1exemplify lipotoxicity to mediate declines of reproductive efficacy of broiler hens. Domest. Anim. Endocrinol. 42(3):183-94.
  37. Pham, M. H., X. H. Tran, Y. P. Lee, D. L. Pham, V. T. Hoang, M. Tixier-Boichard, and C. F. Chen*. 2012. Genetic diversity of the major histocompatibility complex region in Vietnamese local chickens using the LEI0258 microsatellite marker. Vietnamese J. Anim. Sci. Tech. 34:11-19.
  38. Chang, C. S., C. F. Chen, C. Berthouly-Salazar, O. Chazara, Y. P. Lee, C. M. Chang, K. H. Chang, B. Bed’Hom, and M. Tixier-Boichard. 2012. A global analysis of molecular markers and phenotypic traits in local chicken breeds in Taiwan. Animal Genetics 43:172-182.
  39. 劉秀洲、杜宗哲、Christel, Marie-Etancelin、李淵百、陳志峰*。 2012。褐色菜鴨殘差飼料採食量之遺傳參數估算。畜產研究 45:113-140。
  40. Chang, C. S., M. Tixier-Boichard, O. Chazara, Y. P. Lee, C. F. Chen, P. C. Chang, J. W. Chen, and B. Bed’Hom. 2011. Different immune responses to three different vaccines following H6N1 low pathogenic avian influenza virus challenge in Taiwanese local chicken breeds. BMC Proc. 4:S33.
  41. 許萌芳、陳慧文、陳志峰、王金和。2011。2010年台灣部分雞隻家禽白血病J亞群之血清抗體盛行率。台灣獸醫誌 37:227-232。
  42. 蔡文慈、陳志峰、陳慧文、張書維、李建榖、王金和。2011。台灣土雞家禽網狀內皮增生症病毒之感染情形。台灣獸醫誌 37:143-148。
  43. Dorshorst, B., A.M. Molin, A. Johansson, L. Stromstedt, M. H. Pham, C. F. Chen, F. Hallbook, C. Ashwell, and L. Andersson. 2011. A complex genomic rearrangement involving the Endothelin 3 locus causes dermal hyperpigmentation in the chicken. PLoS Genet. 7(12):e1002412.
  44. Wu, J. R., Y. R. Wu, J. H. Shien, Y. M. Hsu, C. F. Chen, H. K. Shieh, and P. C. Chang. 2011. Recombinant proteins containing the hypervariable region of the haemagglutinin protect chickens against challenge with Avibacterium paragallinarum. Vaccine. 29(4):660-667.
  45. Lin, Y. P., L. R. Chen, C. F. Chen, J. F. Liou, Y. L. Chen, J. R. Yang, and Y. L. Shiue. 2010. Identification of early transcripts related to male development in chicken embryos. Theriogenology 74:1161-1178.
  46. Chen, L. R., S. C. Lee, Y. P. Lin, Y. L. Hsieh,Y. L. Chen, J. R. Yang, J. F. Liou, C. F. Chen, Y. P. Lee, and Y. L. Shiue. 2010. Prostaglandin-D synthetase induces transcription of the LH beta subunit in the primary culture of chicken anterior pituitary cells via the PPAR signaling pathway. Theriogenology 73:367-382.
  47. Chen, C. F., Y. H. Chen, M. Tixier-Boichard, P. Y. Cheng, P C. Tang, C. S. Chang, and Y. P. Lee. 2009. Effects of chicken sex-linked dwarf gene on growth and muscle development. Asian-Aust. J. Anim. Sci. 22:937-942.
  48. Chen J. P. W., H. Y. Chan, C. F. Chen, K. C. Tung, and C. C. Chang. 2009. The reproductive performance of female Formosan sambar deer (Cervus unicolor swinhoei) in semi-domesticated herds. Theriogenology, 71:1156-1161.
  49. Chen, C. F., D. Gourichon, N. Z. Huang, Y. P. Lee, A. Bordas, and M. Tixier-Boichard. 2009. Performance comparison of dwarf laying hens segregating for the naked neck gene in temperate and subtropical environments. BMC Genet. Sel. Evol. 41:13.
  50. Chen, C. F., N. Z. Huang, D. Gourichon, Y. P. Lee, M. Tixier-Boichard, and A. Bordas. 2008. Effect of introducing the naked neck gene in a line selected for low residual feed consumption, on performance in temperate or subtropical environments. Poultry Sci. 87:1320-1327.
  51. Berthouly, C., B. Bed’Hom, M. Tixier-Boichard, C. F. Chen, Y. P. Lee, D. Laloe, H. Legros, E. Verrier, X. Rognon. 2008. Using molecular markers and multivariate methods to study the genetic diversity on local European and Asian chickens breeds. Animal Genetics 39:121-129.
  52. Chen, C. F.*, Y. L. Shiue*,C. J. Yen, P. C. Tang, and Y. P. Lee. 2007. Laying traits and underlying transcripts, expressed in the hypothalamus and pituitary gland, that were associated with egg production variability in chickens. Theriogenology 68:1305-1313.
  53. Yang, K.T., C. Y. Lin, H..L. Huang, J. S. Liou, C. Y. Chien, C. P. Wu, C. W. Huang, B. R. Ou, C. F. Chen, Y. P. Lee, EC Lin, P. C. Tang, W. C. Lee, S. T. Ding, W. T. K. Cheng and M. C. Huang. 2007. Expressed transcripts associated with high rates of egg production in chicken ovarian follicles. Molecular and Cellular Probes 22:47-54.
  54. Ding, S. T., Y. H. Ko, B. R. Ou, P.H. Wang, C.L. Chen, M. C. Huang, Y. P. Lee, E.C. Lin, C. F. Chen, H.W. Lin, and W. T. K. Cheng. 2008. The expression of genes related to egg production in the liver of Taiwan Country chickens. Asian-Aust. J. Anim. Sci. 21:19-24.
  55. Chen, Y. H. C. F. Chen, M. Tixier-Boichard, Y. P. Lee. 2007. The effect of sex-linked dwarf gene on meat quality of chickens. J. Chin. Soc. Anim. Sci. 36:115-125.
  56. Wu, J. R., J. H. Shien, H. K. Shieh, C. F. Chen, P. C. Chang. 2007. Protective immunity conferred by recombinant Pasteurella multocida lipoprotein E (PlpE). Vaccine 25:4140-4148.
  57. Yang K. T., C. Y. Lin, J. S. Liou, Y. H. Fan, S. H. Chiou, C. W. Huang, C. P. Wu, E. C. Lin, C. F. Chen, Y. P. Lee, W. C. Lee, S. T. Ding, W. T. K. Cheng, and M. C. Huang. 2007. Differentially expressed transcripts in shell glands from low and high egg production strains of chickens using cDNA microarrays. Anim. Reprod. Sci.101(1-2):113-124.
  58. Chen, L. R., C. H. Chao, C. F. Chen, Y. P. Lee, Y. L. Chen, Y. L. Shiue. 2007. Expression of 25 high egg production related transcripts that identified from hypothalamus and pituitary gland in red-feather Taiwan country chickens. Anim. Reprod. Sci. 100:172-185.
  59. Shiue, Y. L., L. R. Chen, C. F. Chen, J. P. Ju, C. H. Chao, Y. P. Lin, Y. M. Kuo, P. C. Tang, and Y. P Lee. 2006. Identification of transcripts related to high egg production in the chicken hypothalamus and pituitary gland. Theriogenology, 66:1274-1283.
  60. Yang K.T., C.Y. Lin, C.Y. Chien, C.P. Wu, C.W. Huang, B.R Ou, C.F. Chen, Y.P Lee, W.C. Lee, E.C. Lin, S.T. Ding, W.T.K. Cheng and M.C. Huang. 2006. Effects of relative humidity, dot space, printing solution and slide coating on the dot morphology of gene chips. J. Chinese Soc. Anim. Sci. 35:145-151.
  61. Chao, C. H., Y. M. Huang, C. F. Chen, Y. C. Ho, M. L. Su, Y. P. Lee. 2005. The egg production of commercial breeds of Taiwan Country Chicken. J. Chin. Soc. Anim. Sci. 34: 151-161.
  62. Chao, C. H., M. J. Lin, Y. L. Lai, M. L. Su, Y. C. Ho, C. F. Chen, Y. P. Lee. 2005. The growth performances of commercial red-feathered and black-feathered Taiwan Country Chicken. J. Chin. Soc. Anim. Sci. 34: 65-77.
  63. Kuo, Y. M., Y. L. Shiue, C. F. Chen, P. C. Tang, and Y. P Lee. 2005. Proteomic analysis of hypothalamic proteins of high and low egg production strains of chickens. Theriogenology 64:1490-1502.
  64. Fan, Y.K., J. C. Ju, S. L. Lee, C. F. Chen, H. C. Peh, J. C. Hsu, and Y. P. Lee. 2004. High ejaculation frequency enhances production of semen in Taiwan Country Chicken. Asian-Aust. J. Anim. Sci. 17:924-929.
  65. Chen, C. F., A. Bordas, D. Gourichon, and M. Tixier-Boichard. 2004. Effect of high ambient temperature and naked neck genotype on performance of dwarf brown-egg layers selected for improved clutch length. Br. Poult. Sci. 45:346-354.
  66. Tixier-Boichard M., C. F. Chen, A. Schobert, T. Heirman, T. Garcia Lara. 2003. Genetic variability of clutch length in dwarf layers: prospects for poultry breeding. Br. Poult. Sci. 44:798-800.
  67. Chen, C. F., and M. Tixier-Boichard. 2003. Correlated response to long-term selection for clutch length in dwarf brown-egg layers carrying or not the naked neck gene. Poultry Sci. 82:709-720.
  68. Chen, C. F., and M. Tixier-Boichard. 2003. Estimation of genetic variability and selection response for clutch length in dwarf brown-egg layers carrying or not naked neck gene. Genet. Sel. Evol. 35:219-238.
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