Laboratory of Animal Proteomics

 

I. Laboratory Introduction:
This Lab. was established in August 2006. The major missions were to apply molecular genetics and proteomics related technical platform in studying and developing reproduction related molecular markers in livestock and poultry. The Lab. also participate in The iEGG and Animal Biotechnology Center.
The principal investigator is Dr. San-Yuan Huang. The Lab. currently has six master student, and two undergraduate students.



The Lab. members

Introduction to Lab. members

I. Graduate students
1. Pantira Tungsirikul (PET)
Tel: (+886) 0986841726
E-mail: pantira.tung@gmail.com
Education:
Senior high school, Sarasas Witaed Bangbuathong School in Bilingual Program.
Bachelor of Animal Science and Agricultural Technology, Silpakorn University.
2021/2-  Graduate student, Department of Animal Science, National Chung Hsing University.
Personal interest: Travel
 
2. Wei-Tse Hung
Tel: +886-975862396
E-mail:axsz951475321
Education:
Department of Animal Science Studying, National Chung Hsing University.
2021/9-   Graduate student, Department of Animal Science Studying, National Chung Hsing University.
Personal interest: Badminton
 
3. Hsin-Yi Lin
Tel: 0985117208
E-mail: lindidi39@gmail.com
Education:
Department of Livestock Products & Hygienics, The Affiliated Taichung Agricultural Senior High School of National Chung Hsing University
Department of Animal Science, National Pingtung University of Science and Technology (NPUST)
2021/9-   Graduate student, Department of Animal Science Studying, National Chung Hsing University.
Working experiences:
Livestock Research Institute, COA - Hengchun Branch
GeneReach Biotechnology Crop.
Experimental skills: PCR-RFLP
Personal interest:  trip and watch drama
 
4. Shun-Jen Liu
Tel: 0988125259
E-mail: jm7020@hotmail.com
Education:  
Taoyuan Municipal Nei-Li Senior High School
Department of Animal Science, National Chung Hsing University
2021/9-  Professional Master Program of Agricultural Business Management, NCHU
Working experiences:
Pig Farm    2012-2013
Animal Technology Laboratories   Assistant Researcher 2013-2014
Agricultural Technology Research Institute   Assistant Researcher  2014-now
Experimental skills: 
Pig flow
Sow management
Personal interest: Jogging, watch a movie, tabletop game

5. Shu-Ping Chang
Tel: +886-4-22870613 ext.275; mobile phone: 0972-867526
E-mail: nykiri042234@gmail.com
Education:
Department of Animal Science, National Chung Hsing University
Personal interest: watching medical dramas、detective series dramas; playing volleyball; chatting(?)

6. Ta-Fu Lin
Tel: +886-4-22870613 ext.275
E-mail: davelin465@gmail.com
Education:
Department of Animal Science, National Chung-Hsing University.
Personal interest: Ride a motorcycle, dancing, listening to music, running
  
II. Undergraduates
1. Jia-Huei Chen
Tel: +886-4-22870613 ext.275
E-mail: hellen891002@gmail.com
Education:
Taichung Municipal Dali Senior High School
Department of Animal Science, National Chung-Hsing University.
Personal interest: Painting, Reading, Horse riding

2. En-Jui Liu
Tel: +886-4-22870613 ext.275
E-mail: ejliu888@gmail.com
Education:
Taipei Municipal Zhong-Lun High School.
Department of Animal Science, National Chung Hsing University.
Personal interest: Badminton, Volley ball, animation, piano, travel.

 

 

II. Research topic
1. Transcriptomic and proteomic study on the termotolerace of Taiwan country chickens

 

2D-DIGE protein profile of the SYFs of layer-type L2 strain Taiwan country chickens. Annotation of differentially expressed proteins in the SYFs of layer-type L2 strain Taiwan country chickens after acute heat stress.

(Cheng et al., 2018)


2. Study on the genome-wide single nucleotide polymorphisms (SNPs) in Taiwan country chickens and different breeds of pigs

Manhattan plot of the observed P values for body temperature change in the genome-wide association study between resistant vs. susceptible groups of broiler-type B strain Taiwan country chickens.

 

 

3. Others
Applying proteomics-based platforms to investigate responses to nanomaterial, footpad dermatitis-related proteins, and phosphoproteome in sperms of chickens.
 
III. Important equipment in the Lab.
The Lab. possesses basic equipment required for proteomic analysis (including sample preparation, two-dimensional gel electrophoresis, gel scanning, and image analysis), phase contrast microscope equipped with photography software, and two climate chambers. The high-end equipment for proteomic study were achieved through cooperation with Labs in National Tsing-Hua University and China Medical University.

 

Important equipment in Laboratory of Animal Proteomics. A: IPGphor IEF unit; B: Gel Tank for protein analysis; C: Low temperature centrifuge; D: Phase contrast microscope; E: Sonicator; F: Gel scanner; G: Speed-vac centrifuge; H: Climate chamber for chickens.


IV.Recent publications:

  1.  Yuan, C.W. #, J.T. Huang#, C.C. Chen, P.C. Tang, J.W. Huang, J.J. Lin, S.Y. Huang*, S.E. Chen*. 2017. Evaluation of efficacy and toxicity of exfoliated silicate nanoclays as a feed additive for fumonisin detoxification. J. Agri. Food Chem. 65:6564-6571. doi: 10.1021/acs.jafc.7b02224# These authors contributed equally.
  2. Yu, Y.C., D.N. Lee, H.L. Li, and S.Y. Huang. 2017. Effect of single nucleotide polymorphisms in E. coli F4ab/ac resistance related genes on the growth traits of pigs. J. Chin. Soc. Anim. Sci. 46:321-330.
  3. Cheng, C.Y., W.L. Tu, C.J. Chen, H.L. Chan, C.F. Chen, H.H. Chen, P.C. Tang, Y.P. Lee, S.E. Chen, and S.Y. Huang. 2018. Functional genomics study on the acute heat stress response in small yellow follicles of a layer-type chickens. Sci. Rep. 8:1320. doi:10.1038/s41598-017-18335-5
  4. Cheng, C.Y., W.L. Tu, , C.J. Chen, H.L. Chan, C.F. Chen, H.H. Chen, P.C. Tang, Y.P. Lee, S.E. Chen, and S.Y. Huang. 2018. Proteomic analysis of thermal regulation of small yellow follicles in boiler-type Taiwan country chickens. J. Poult. Sci. 55:120-136.
  5. Tu, W.L., C.Y. Cheng, C.J. Chen, H.L. Chan, S.H. Wang, P.C. Tang, C.F. Chen, H.H. Chen, Y.P. Lee, S.E. Chen*, and S.Y. Huang*. 2018. Proteomic analysis of the hypothalamus of broiler-type Taiwan country chickens in response to acute heat stress. Anim. Sci. J. 89:1475-1485.
  6. Tu, W.L., C.Y. Cheng, C.J. Chen, H.L. Chan, S.H. Wang, P.C. Tang, C.F. Chen, Y.P. Lee, S.E. Chen, and S.Y. Huang*. 2018. Annotation of differential protein expression in the hypothalami of layer-type Taiwan country chickens in response to acute heat stress. J. Thermal Biol. 77:157-172.
  7. Zhuang, Z.X., S.C. Chang, C.J. Chen, H.L. Chan, M.J. Lin, H.Y. Liao, C.Y. Cheng, T.Y. Lin, Y.S. Jea, and S.Y. Huang. 2019. Effect of seasonal change on testicular protein expression in White Roman geese. Anim. Biotechnol. 30:43-56. doi: 10.1080/10495398.2018.1432488
  8. Chen, T.M., M.C. Lai, Y.H. Li, Y.L. Chan, C.H. Wu, Y.M. Wang, C.W. Chien, S.Y. Huang, H.S. Sun⁠⁎, and S.J. Tsai*. 2019. ⁠hnRNPM induces translation switch under hypoxia to promote colon cancer development. EBioMedicine 41:299-309. DOI: https://doi.org/10.1016/j.ebiom.2019.02.059
  9.  Zhuang, Z.X., S.E. Chen, C.F. Chen, E.C. Lin, and S.Y. Huang*. 2019. Genome-wide association study on the body temperature changes of a broiler-type strain Taiwan country chickens under acute heat stress. J. Thermal Biol. 82:33-42.
  10.  Wang, S.H.#, C.Y. Cheng#, C.J. Chen, H.L. Chan, H.H. Chen, P.C. Tang, C.F. Chen, Y.P. Lee, and S.Y. Huang*. 2019. Acute heat stress changes protein expression in the testes of a broiler-type strain of Taiwan country chickens. Anim. Biotechnol. 30:129-145. doi=10.1080/10495398.2018.1446972# These authors contributed equally.
  11.  Zhuang, Z.X., S.E. Chen, C.F. Chen, E.C. Lin*, and S.Y. Huang*. 2020. Genomic regions and pathways associated with thermotolerance in layer-type strain Taiwan indigenous chickens. J. Thermal Biol. 88: 102486. https://doi.org/10.1016/j.jtherbio.2019.102486
  12.  Zheng, H.T., Z.X. Zhuang, C.J. Chen, H.-Y. Liao, H.L. Chen, H.C. Hsueh, C.F. Chen, S.E. Chen*, and S.Y. Huang*. 2021. Effect of acute heat stress on protein expression and histone modification in the adrenal gland of male layer-type country chickens. Sci. Rep. 11:6499. doi: 10.1038/s41598-021-85868-1
  13. Wu, C.H., W. Chumngoen, C.J. Chen, D.Y. Lin, H.Y. Liao, F.J Tan*, and S.Y. Huang*. 2021. Differential protein expression in Biceps femoris muscles with different tenderness in Taiwan country chickens. J. Chin. Anim. Sci. 50:137-174.
  14. Zhuang, Z.X., S.E. Chen, C.F. Chen, E.C. Lin, and S.Y. Huang*. 2022. Single-nucleotide polymorphisms in genes related to oxidative stress and ion channels in chickens are associated with semen quality and hormonal responses to thermal stress. J. Thermal Biol. 105:103220. doi: 10.1016/j.jtherbio.2022.103220.