| 報告時間：2025-12-26 |
| 報告地點：Room 407 |
| 指導老師： Dr. San-Yuan Huang, Chao-Jung Chen |
| 學生：Yue-Cih Peng |
| 摘要 |
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Spent coffee grounds (SCG) cause pollution if discarded. With the rise of the circular economy, the application of SCG has gradually gained attention. SCG contains phenolic and caffeine compounds. Solid-state fermentation of SCG with Aspergillus oryzae may enhance the bioactive components and nutritional value of SCG. When added to feed, the SCG solid state fermentation products (SCG-SFP) may promote beneficial microbial growth in intestine, improve intestinal structure, and potentially alter blood metabolites of chickens. The study aimed to investigate the effects of dietary SCG-SFP supplementation on liver proteome and serum metabolome in commercial red–feathered Taiwan country chickens. A total of 240 one-day-old chicks were randomly assigned to four treatments: Control group (CT) fed a basal diet, low-dose group (L) fed a basal diet supplemented with 1 g/kg feed of SCG-SFP, high-dose group (H) fed a basal diet supplemented with 2 g/kg feed of SCG-SFP, and antibiotic group (AL) fed a basal diet supplemented with 10 ppm Avilamycin. At 84 days of age, eight chickens from each group (four males and four females) were randomly selected for liver and blood sampling. Liver proteome analysis was performed using nano-flow UHPLC coupled with a quadrupole-Orbitrap mass spectrometer (nanoUHPLC-ESI-Orbitrap 480). Differentially expressed proteins (DEPs) were identified based on the criteria of fold change (FC) ≥ 1.5 or ≤ 0.67 and P < 0.05. Functional annotation and classification of the DEPs were analyzed by using gene ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) databases. Serum metabolites were analyzed by LC‑MS/MS (QTRAP™ 6500+) and statistically analyzed with SCIEX OS software. Significantly differred metabolites (FC ≥ 1.2 or ≤ 0.83, P < 0.05) were analyzed using MetaboAnalyst 6.0 and KEGG. Results revealed that there were 4,093 proteins identified in the liver proteome. The were 78 proteins upregulated and 42 proteins downregulated between the H and CT groups while there were 21 proteins upregulated and 30 proteins downregulated between the H and AL groups. GO analysis indicated that these DEPs were mainly involved in active transmembrane transporter activity, regulation of oxidoreductase activity, organic acid biosynthesis, fatty acid metabolism, respiratory electron transport chain, and fatty acid biosynthesis. KEGG pathway analysis revealed that the DEPs were enriched in PPAR signaling, oxidative phosphorylation, and glycerophospholipid metabolism. Targeted serum metabolomic analysis showed that there were 11 metabolites increased and 6 decreased in H group when compared with CT group. These differential metabolites were mainly linked to caffeine metabolism. Elevated caffeine, 1,7-dimethylxanthine, theobromine, and theophylline suggested higher energy demand. The reduced cortisone and meso erythritol associated with increased glycerol and melatonin indicated enhanced hepatic fatty acid metabolism. In conclusion, SCG-SFP supplementation increased energy demand in chickens through caffeine and other bioactive compounds. SCG-SFP upregulated hepatic fatty acid β-oxidation and improved efficiency of mitochondrial respiratory chain to generate ATP without compromising growth performance and thereby supporting the health of chickens. These findings highlight the potential of SCG-SFP as a sustainable alternative to antibiotics in chicken production. |
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