| 報告時間：2026-09-16 | |
| 報告地點：大四教室 | |
| 指導老師：陳洵一 | |
| 學生：陳艾語 | |
| 摘要 | |
| The gut microbiota plays important roles in nutrient utilization, intestinal development, immune regulation, and host health. However, the origin and early establishment of the avian gut microbiota remain controversial. Newly hatched pigeon squabs depend on pigeon milk feeding by their parents, and thereby pigeon milk may contribute to the establishment of the squab gut microbiota. This study investigated the early development of the gut microbiota in White King pigeon squabs in respect to pigeon-milk microbiota. 16S rRNA gene sequencing with fecal samples collected from squabs from 0 to 21 days(F0, F7, F14, and F21) and pigeon-milk samples from the crops of squabs from 0 to 21 days after parental feeding (M0, M7, M14, and M21) was employed to characterize the dynamics of bacterial communities. Diverse bacterial DNA was detected in the meconium, with Enterococcus, Prevotella_9, and Acinetobacter as the dominant genera. However, the fecal microbial community changed rapidly during the first week after hatching and followed by the dominant genera included Lactobacillus, Limosilactobacillus, and Turicibacter. Principal coordinate analysis clearly separated F0 from the post-feeding fecal groups, whereas F7, F14, and F21 clustered more closely together, suggesting relatively stable microbiota after the first week. The pigeon-milk microbiota was dominated by Limosilactobacillus, Ligilactobacillus, Lactobacillus, Bifidobacterium, and Aeriscardovia, accounting for approximately 90% of the total abundance. These five genera represented only 1.125% of the microbial abundance in F0 but increased to over 70% after F7. Meconium showed no abundance correlation with later fecal samples, whereas colostrum showed very strong positive correlations with fecal microbiota with Pearson correlation coefficients ranging from 0.843 to 0.922. In conclusion, microbial DNA was detectable in newly hatched squabs, but the fecal microbial community changed substantially after pigeon-milk consumption. The strong similarity between colostrum and post-feeding fecal microbiota suggests that pigeon milk may play an important role in early gut microbiota establishment. Further bacterial culture and strain-level tracking are therefore required to verify microbial transmission.
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